Inositol 1,4,5-trisphosphate 3-kinase activity in high-speed supernatants from rat pancreatoma cells, AR4-2J
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چکیده
منابع مشابه
Inositol (1,4,5) trisphosphate 3 kinase B controls positive selection of T cells and modulates Erk activity.
The mechanisms governing positive selection of T cells in the thymus are still incompletely understood. Here, we describe a N-ethyl-N-nitrosourea induced recessive mouse mutant, Ms. T-less, which lacks T cells in the peripheral blood because of a complete block of thymocyte development at the CD4(+)CD8(+) stage. Single nucleotide polymorphism mapping and candidate gene sequencing revealed a non...
متن کاملPurine-based inhibitors of inositol-1,4,5-trisphosphate-3-kinase.
Inositol 1,4,5-trisphosphate 3-kinase (IP3K) catalyzes the adenosine triphosphate (ATP) dependent phosphorylation of inositol 1,4,5-trisphosphate (IP3) to inositol 1,3,4,5-tetrakisphosphate (IP4) and plays an important role in the regulation of intracellular calcium concentration. IP3 is a well-known second messenger that stimulates intracellular calcium mobilization, which is in turn responsib...
متن کاملHuman brain inositol 1,4,5-trisphosphate 3-kinase cDNA sequence.
A human brain cDNA encoding inositol 1,4,5-trisphosphate 3-kinase was isolated by screening a human hippocampus cDNA hbrary constructed in the bactenophage lambda ZapII (Stratagene) with a partial rat brain cDNA probe (1). The cDNA sequence was deduced from two overlapping clones after subcloning in M13 and sequencing on both strands. The human and rat sequences presented 87% homology in the co...
متن کاملInhibition of inositol 1,4,5-trisphosphate 3-kinase by heparin: basal and Ca2+/calmodulin-stimulated activity.
Inositol 1 ,4,5-trisphosphate (InsP3) 3-kinase is responsible for the synthesis of InsP4. The kinase in many tissues is regulated by Ca2+/calmodulin (CaM; see [1] for review). The activity of purified enzyme from bovine brain is stimulated up to 30-fold by Ca2+/CaM. Stimulation by CaM appears to result from an increase in enzyme maximal velocity without a substantial change of Km for InsP3 [2]....
متن کاملInositol trisphosphate metabolism in Saccharomyces cerevisiae: identification, purification and properties of inositol 1,4,5-trisphosphate 6-kinase.
Ins(1,4,5)P3 metabolism was examined in Saccharomyces cerevisiae extracts. S. cerevisiae contains readily detectable Ins(1,4,5)P3 kinase activity that is predominantly soluble, but phosphomonoesterase activity acting on Ins(1,4,5)P3 was not detected in either soluble or particulate preparations from this organism. We have purified the kinase activity approximately 685-fold in a rapid four-step ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1991
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2740622